Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments
Abstract
Fragments of DNA from derivatives of phage λ having either one or two targets for R.EcoRI have been joined in new combinations to give biologically active phage genomes. These include two classes of deletion mutants; in one, only the DNA between the targets is deleted, but in the second the deletions are more extensive. Other fragments of DNA have been inserted into these phage chromosomes.
- Publication:
-
Nature
- Pub Date:
- October 1974
- DOI:
- 10.1038/251476a0
- Bibcode:
- 1974Natur.251..476M