Glucose Naturally Labeled with Carbon-13: Use for Metabolic Studies in Man
Abstract
The ratio of carbon-13 to carbon-12 is much higher in most commercial preparations of glucose used for oral glucose tolerance tests than it is in carbon dioxide in expired air. This recent discovery provided a novel and potentially significant means of studying glucose metabolism. The changes in the ratio of carbon-13 to carbon-12 in carbon dioxide expired after oral glucose administration were determined by mass spectrometry. In six healthy male volunteers, the administration of glucose resulted in a marked, reproducible rise in the isotopic ratio in expired carbon dioxide; the ratio reached its maximum at 4 hours and then declined progressively.
- Publication:
-
Science
- Pub Date:
- August 1973
- DOI:
- 10.1126/science.181.4098.445
- Bibcode:
- 1973Sci...181..445L