The Role of 1,N6-Ethenoadenosine Triphosphate and 1,N6-Ethenoadenosine Monophosphate in Firefly Luminescence
Abstract
1,N6-Ethenoadenosine triphosphate (ɛ-ATP) has been found to be inactive as a substrate for firefly luciferase. However, chemically synthesized luciferyl-ɛAMP is oxidized by luciferase and light is emitted. The color of the light is red in contrast to the normal yellow-green observed with luciferyladenylate. Although ɛATP will not serve as a substrate in the activation reaction, the reversal of the activation, pyrophosphorolysis of dehydroluciferyladenylate, will occur with the ethenoadenosine analog.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- June 1973
- DOI:
- 10.1073/pnas.70.6.1664
- Bibcode:
- 1973PNAS...70.1664D