Thermodynamic Fluctuations in a Reacting System-Measurement by Fluorescence Correlation Spectroscopy
Abstract
The temporal correlations of thermodynamic concentration fluctuations have been measured in a chemically reactive system at equilibrium by observing fluctuations of the fluorescence of a reaction product. The experiment yields the chemical rate constants and diffusion coefficients and shows the coupling among them. Data are reported for binding of ethidium bromide to DNA.
- Publication:
-
Physical Review Letters
- Pub Date:
- September 1972
- DOI:
- 10.1103/PhysRevLett.29.705
- Bibcode:
- 1972PhRvL..29..705M