Binding of Thymus- and Bone Marrow-Derived Lymphoid Cells to Antigen-Derivatized Fibers
Abstract
Thymus-derived lymphocytes (T cells) and bone marrow-derived lymphocytes (B cells) from mouse spleens bind specifically to antigen-derivatized nylon fibers. The fiber-bound population consisted of about 60%-70% B cells and 30% T cells as determined by cytotoxicity, fluorescence, and antibody-complement binding assays. Essentially all fiber-bound cells were viable and could be accounted for as T or B cells. Enriched populations of T or B cells could be isolated on the fibers by destruction of one or the other cell type with the appropriate antiserum plus complement. T or B cells could also be fractionated according to their relative affinity (or avidity) for a given antigen.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 1972
- DOI:
- 10.1073/pnas.69.12.3774
- Bibcode:
- 1972PNAS...69.3774R