Inactivation of Ribosomes In Vitro by Colicin E3
Abstract
Cell-free protein synthesizing extracts incubated with purified preparations of colicin E3 showed a marked decrease of their protein synthesizing activity. Incubation of ribosomes prepared from S-30 extracts with E3 resulted in their inactivation. Their 16S RNA was found to lose a terminal fragment, similar in size to the fragment released upon in vivo treatment with E3. Extracts derived from colicinogenic strains are resistant to the action of E3in vitro.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- October 1971
- DOI:
- 10.1073/pnas.68.10.2421
- Bibcode:
- 1971PNAS...68.2421B