Phosphorylation of Ribosome-Associated Protein by an Adenosine 3':5'-Cyclic Monophosphate-Dependent Protein Kinase: Location of the Microsomal Receptor and Protein Kinase
Abstract
In the adrenal cortex, the adenosine 3‧:5‧-cyclic monophosphate (cAMP)-receptor protein and the cAMP-dependent protein kinase are located in both the cytosol and endoplasmic reticulum. The cAMP-dependent protein kinase from the cytosol catalyzes the phosphorylation of ribosome-associated protein. Ribosomes washed in 0.5 M NH4Cl retain the substrate of the protein kinase reaction, but are dependent on the NH4Cl extract for in vitro protein synthesis. Dissociation of the 80S ribosomes by 0.88 M KCl, however, releases the ribosome-associated (protein) substrate of the phosphorylation reaction.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- May 1971
- DOI:
- 10.1073/pnas.68.5.880
- Bibcode:
- 1971PNAS...68..880W