Karyotyping of Single Human Chromosomes from Dry Mass Determined by Electron Microscopy
Abstract
Human chromosomes from cultures of peripheral lymphocytes were prepared for electron microscopy by surface spreading and critical-point drying. Total and partial determinations of chromosomal dry mass were made from contrast in electron microscopic negatives. The distribution of dry weights was analyzed with respect to the Denver-Chicago classifications. There is considerable variability of mass within each group, but no statistically significant differences between four donors. A single chromosome can be correctly classified from its total mass and the ratio of long- to short-arm mass.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- April 1971
- DOI:
- 10.1073/pnas.68.4.726
- Bibcode:
- 1971PNAS...68..726B