The Reconstruction of a ThreeDimensional Structure from Projections and its Application to Electron Microscopy
Abstract
A transmission electron micrograph is essentially a projection of the specimen in the direction of view. In order to reconstruct a threedimensional image of the specimen, it is necessary to be able to combine data from a number of different views. A formal solution of this problem is given in terms of Fourier transforms. Its realization requires data reduction and interpolation. The final solution is given by a least squares approach, which also indicates how many views must be included to give a valid reconstruction of a given particle to a given degree of resolution. Interpolation procedures of varying power are given, to be employed according to the economy with which the available data must be used. An alternative procedure is described for direct reconstruction without the use of Fourier transforms, but it is shown to be in general less practicable than the Fourier approach.
 Publication:

Proceedings of the Royal Society of London Series A
 Pub Date:
 June 1970
 DOI:
 10.1098/rspa.1970.0119
 Bibcode:
 1970RSPSA.317..319C