DNA Synthesis by RNA-Containing Tumor Viruses
Abstract
Murine leukemia (Rauscher and Moloney strains) and sarcoma (Kirsten strain) virions, as well as the mammary tumor virus of mice, contain an RNA-dependent DNA polymerase. Optimal incorporation of deoxyribonucleoside triphosphates occurs at a critical detergent (Triton X-100) concentration (0.010-0.014%). At higher than optimal detergent concentrations the virion is seen to be disrupted and enzyme activity is lost. The virion, enzymatic activity, and newly synthesized DNA all cosediment in a sucrose gradient. Thus far the enzymatic activity has been found only in RNA viruses that have oncogenic properties.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- October 1970
- DOI:
- 10.1073/pnas.67.2.1034
- Bibcode:
- 1970PNAS...67.1034S