A Protein Binding Assay for Adenosine 3':5'-Cyclic Monophosphate
Abstract
A simple and sensitive assay for adenosine 3‧:5‧-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10-9 M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- September 1970
- DOI:
- 10.1073/pnas.67.1.305
- Bibcode:
- 1970PNAS...67..305G