Mapping of a Human Genetic Regulator Element by Somatic Cell Genetic Analysis
Abstract
The ES-2 esterase is a kidney-associated esterase and is expressed in a cloned mouse renal adenocarcinoma cell line. Somatic cell hybridization of the mouse renal adenocarcinoma with either mouse or human fibroblasts leads to the marked reduction of ES-2 esterase titer. The extinction of ES-2 esterase activity in mouse×human hybrids is reversible. Extinction correlates with the presence of the human C10 chromosome in the somatic cell hybrids; whereas reexpression of the ES-2 esterase is observed in hybrids which have lost C10. Thus, the proposed regulator element involved is probably structurally linked to C10; however, linkage to a member of the D or G groups cannot be completely excluded.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- August 1970
- DOI:
- 10.1073/pnas.66.4.1220
- Bibcode:
- 1970PNAS...66.1220K