Peptide Chain Termination, VI. Purification and Site of Action of S
Abstract
Peptide chain termination is a result of at least two events: terminator codon recognition and hydrolysis of peptidyl tRNA. A protein factor S, isolated from the supernatant of Escherichia coli B, stimulates fMet release. Factor S lowers the Km for terminator trinucleotides without altering the Vmax of release and therefore acts at terminator codon recognition. The S protein differs from initiation factors, elongation factor G, several forms of elongation factor T and release factors. The importance of the 2% Tu content in purified S is unresolved.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- February 1970
- DOI:
- 10.1073/pnas.65.2.430
- Bibcode:
- 1970PNAS...65..430G