Codon Recognition by Enzymatically Mischarged Valine Transfer Ribonucleic Acid
Abstract
Direct evidence for the adaptor hypothesis has been obtained by examining the codon recognition of a purified Escherichia coli valine transfer ribonucleic acid which was enzymatically mischarged with phenylalanine labeled with carbon-14 by reaction with purified phenylalanyl-transfer ribonucleic acid synthetase from Neurospora crassa. The mischarged transfer ribonucleic acid recognized the valine codons but failed to recognize the phenylalanine codon when tested in trinucleotide-directed ribosomal binding assay.
- Publication:
-
Science
- Pub Date:
- December 1969
- DOI:
- 10.1126/science.166.3913.1635
- Bibcode:
- 1969Sci...166.1635G