ISOLATION OF ADENYL CYCLASE FROM Escherichia coli

We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg⁺⁺ requirement and is inhibited by fluoride and inorganic pyrophosphate.