A Template-Selective Inhibitor of in vitro Transcription
Abstract
A macromolecular factor, TF1, has been isolated from bacteriophage SP01-infected B. subtilis. TF1 selectively inhibits in vitro transcription of SP01 and related viral DNA. Evidence is presented regarding these properties of the repressor-like TF1: (1) template and conformational specificity; (2) interaction with DNA rather than RNA polymerase; (3) trypsin sensitivity; (4) reversibility of action; and (5) ability to block initiation, but not propagation, of RNA synthesis.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- February 1969
- DOI:
- 10.1073/pnas.62.2.514
- Bibcode:
- 1969PNAS...62..514W