DNA-Dependent RNA-Directed Protein Synthesis in vitro, I. Extent of Genome Transcription
Abstract
We have described an in vitro system which couples UTP incorporation into RNA by DNA-dependent RNA polymerase with RNA-directed amino acid incorporation into polypeptides. Using the technique of DNA-RNA hybridization, we have demonstrated that the whole ΦX-174 genome is transcribed during concomitant protein synthesis.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- February 1969
- DOI:
- 10.1073/pnas.62.2.483
- Bibcode:
- 1969PNAS...62..483B