Enzymatic Cleavage and Repair of Transforming DNA
Abstract
Single-strand nicks caused by DNase I are capable of inactivating B. subtilis transforming DNA. Under suitable conditions, the polynucleotide joining enzyme from E. coli and a similar DPN-requiring activity from B. subtilis can completely repair this damage, restore biological activity, and increase the single-strand molecular weight. The rates of inactivation of a single genetic marker and of a four-marker linkage group suggest that a single-strand nick is inactivating even when far from the site of genetic damage.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- January 1969
- DOI:
- 10.1073/pnas.62.1.289
- Bibcode:
- 1969PNAS...62..289L