Two-dimensional Resolution of Plasma Proteins by Combination of Polyacrylamide Disc and Gradient Gel Electrophoresis

ELECTROPHORETIC migration of proteins depends on their electrophoretic mobility and the resistance of the supporting medium. The latter is an important factor in starch and polyacrylamide gels the action of which as molecular sieves contributes greatly to the resolution of protein mixtures. This is further enhanced in gradient gels^1-6 in which protein molecules are driven through progressively decreasing pores until they are brought to a near dead stop in order of their size; a procedure appropriately called ``pore-limit electrophoresis''⁵.