Isolation and Amino-acid Sequence of β-LPH from Sheep Pituitary Glands
Abstract
SEVERAL adenohypophyseal hormones have been demonstrated to possess in vitro lipotropic activity; these include growth, adrenocorticotropic, thyrotropic, α-melanocyte-stimulating and β-melanocyte-stimulating hormones. Recently, Rudman, Astwood and their colleagues reported the preparation of Fraction H, peptides I and II, and showed these preparations to be lipotropic agents1,2. In the course of investigating a simplified procedure for the isolation of ACTH from sheep pituitary glands, we have obtained a lipotropic peptide3 which is chemically distinct from ACTH and other known adenohypophyseal hormones. In this communication, we wish to describe the isolation of another lipotropic peptide (designated as β-LPH) from sheep pituitaries. The complete amino-acid sequence of β-LPH will also be presented here.
- Publication:
-
Nature
- Pub Date:
- December 1965
- DOI:
- 10.1038/2081093b0
- Bibcode:
- 1965Natur.208.1093H