Resuscitation of Hamsters after Supercooling or Partial Crystallization at Body Temperatures Below 0° C.

A VARIETY of cells and tissues of mammals survive for long periods at the temperature of `dry ice' - 79° C., when frozen in media containing glycerol¹. At the other extreme, Andjus's work on the whole animal shows that, by special methods of cooling and rewarming, rats can be revived from deep body temperatures of about + 0.5° C.². We are now attempting to close the gap between these two lines of work (a) by increasing the size and complexity of the isolated tissue cooled to - 79° C., as, for example, by freezing a whole isolated organ³, and (b) by reducing the whole animal to a body temperature below zero. This communication deals with experiments on the second of these developments.