Brewster differential microscopy

Imaging of transparent samples such as cells is important in the biomedicine field; however, insignificant absorption and weakly scattering limit the imaging contrast of phase objects. Here, we propose and demonstrate Brewster differential microscopy based on simple optical reflection at the glass interface. The combination of spin-orbit interaction of light and the Brewster effect can perform two-dimensional differentiation to the incident light distribution and, thus, achieves isotropic edge-enhanced imaging of pure phase objects, which overcomes the limitation of traditional one-dimensional imaging. Furthermore, by introducing bias retardation, we also reconstruct the original phase distribution. The proposed microscopic imaging mechanism does not involve any complex modulation devices and takes advantages of simple and low-cost structure. The results indicate that our research shows promising applications for nondestructive imaging of biological cells.