Autofluorescent characteristics of Candidatus Brocadia fulgida and the consequences for FISH and microscopic detection
Abstract
Identification of Candidatus Brocadia fulgida by three independent methods. Ca. B. fulgida was autofluorescent with maxima at 288–330nm, 288–478nm and 417–478nm. Detection of ghost cells by autofluorescence, FISH and DAPI staining. Ca. B. anammoxidans was either autofluorescent or Ban162 labelled Ca. B. fulgida. 16S rDNA and hzsA fragments clustered well, whereas hzoAB did not.
- Publication:
-
Systematic and Applied Microbiology
- Pub Date:
- March 2019
- DOI:
- 10.1016/j.syapm.2018.09.002
- Bibcode:
- 2019SyApM..42..135B
- Keywords:
-
- 16S rDNA;
- Hydrazine synthase hzsA;
- Hydrazine oxidase hzo;
- FISH;
- Candidatus Kuenenia stuttgartiensis;
- Candidatus Brocadia fulgida