Sugarcane bagasse saccharification using Aspergillus tubingensis enzymatic cocktail for 2G bio-ethanol production
Abstract
In order to meet the increasing demand of 2G ethanol, sugarcane bagasse (SCB), a low cost by-product of sugarcane industry, can be utilized. However, cost-effective degradation of SCB into constituent fermentable sugars and subsequent ethanolic fermentation remains to be a far-fetched goal. In the present study, cellulase and hemicellulase cocktail produced by Aspergillus tubingensis NKBP-55 was applied for SCB hydrolysis under parametrically optimized conditions. Enzyme treatment (SCB: 7%, enzyme load: 1U = 1 mg) resulted in the liberation of fermentable mono-sugars 20 mg/mL (glucose, xylose and arabinose). Powder X-ray diffraction (PXRD) analysis revealed increase in the crystallinity index from 58.4 to 63.9% as a result of degradation of hemicellulose and amorphous cellulose. SCB hydrolysate was fermented to ethanol (0.415 g/g) by glucose and xylose-fermenting yeast, Candida shehatae NCIM 3501. Maximum ethanol concentration (15.54 ± 0.3 g/L) with 77.9% fermentation efficiency and 0.161 g/L/h productivity was achieved. The present study revealed that A. tubingensis enzyme cocktail can be used for efficient SCB hydrolysis and conversion of resulting hydrolysate into bioethanol using C. shehatae.
- Publication:
-
Renewable Energy
- Pub Date:
- June 2020
- DOI:
- 10.1016/j.renene.2020.01.063
- Bibcode:
- 2020REne..152..653P
- Keywords:
-
- Aspergillus tubingensis NKBP-55;
- Bioethanol;
- Candida shehatae;
- Cellulase;
- Ethanolic co-fermentation;
- Sugarcane bagasse