Unravelling nitrogen removal and nitrous oxide emission from mainstream integrated nitrification-partial denitrification-anammox for low carbon/nitrogen domestic wastewater

Stable supply of nitrite is often a major obstacle for achieving mainstream anammox due to washout failure of nitrite oxidizers (NOB) at low influent ammonia of municipal wastewater. In this study, an integrated nitrification, partial denitrification and anammox (INPDA) as a one-stage mainstream nitrogen removal alternative was established in a low-oxygen sequencing batch biofilm reactor treating synthetic sewage. The overall nitrogen removal and nitrous oxide (N₂O) emission were mainly investigated at 50 mg/L NH₄⁺-N influent with a low carbon/nitrogen (C/N) of 2.5. Continuous operation demonstrated that as high as 98.8% NH₄⁺-N and 94.1% TN were removed in SBBR system. Cyclic experiment verified sequential completion of nitrification, partial denitrification and anammox were responsible for high-rate TN removal. During one typical cycle, the trend of N₂O emission was characterized by firstly rapid rise, then fluctuant decrease followed by rapid decrease and finally slow disappearance. The maximum N₂O emission rate reached up to 6.7 μg/(L·min) occurred at 75 min. High-throughput sequencing revealed the co-existence of nitrifying, denitrifying and anammox species and large detection of key functional genes (Hzs, Hdh, Hao, Nor) in an oxygen-limited SBBR, thereby highly correlating nitrogen removal and N₂O emission characteristics. Nitrogen metabolic pathways analysis further suggest denitratation(NO₃^‑-N to NO₂^‑-N)-based anammox is a main route for mainstream nitrogen removal. Moreover, N₂O might be generated by both hydroxylamine oxidation step in nitrification and also heterotrophic denitrification pathway. The research findings provide more deep understandings of enhanced nitrogen removal and mitigated N₂O footprint from a single mainstream anammox-based system.