Virus Interference. II. Some Properties of Interferon
Abstract
Interferon could be titrated by the amount of interference induced in fragments of chorioallantoic membranes challenged with influenza A virus. Over a ten-fold range, inverse proportion between interferon concentration and haemagglutinin titre reached by the challenge virus was observed. Interferon proved stable at 2 degrees C for 2 weeks. Marked inactivation took place after 1 h at 60 degrees C. Interferon was not measurably sedimented by 100 000 g for 1/2 h. It was held back by gradocol filters of a.p.d. 0.6μ . It was not dialyzable. Interferon was active against influenza A, Sendai, Newcastle disease and vaccinia viruses. It was not neutralized by anti-MEL rabbit serum and only slightly inhibited by pooled human serum rich in complement-fixing antibody to influenza A soluble antigen.
- Publication:
-
Proceedings of the Royal Society of London Series B
- Pub Date:
- September 1957
- DOI:
- 10.1098/rspb.1957.0049
- Bibcode:
- 1957RSPSB.147..268I